Roche LightCycler 96 Real-Time PCR System

Used, tested

₹250,000 + 18% GST

  • Availability: arriving soon
  • Condition: Used, tested
  • Brand: Roche
  • Model: LightCycler 96

Compact, mid-throughput real-time PCR system from Roche.

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Description

About this instrument

Compact, mid-throughput real-time PCR system from Roche. Features a high-precision 96-well Peltier block with gradient capability, 4-color optical detection via fixed fiber optics and CCD camera, and fast run times (<40 min for standard 40-cycle PCR). Ideal for gene expression, genotyping, HRM, and multiplex qPCR applications. Standalone touchscreen operation with intuitive software.

Specifications

Brand Roche
Model LightCycler 96
Condition Used, tested
Format 96-well block (supports 96-well plates and 8-tube strips)
Reaction volume 10–50 µL
Thermal system Peltier-based silver block
Max heating rate 4.4 °C/s
Average cooling rate 2.2 °C/s
Temperature range 37–98 °C
Temperature accuracy ±0.2 °C
Temperature uniformity Range (max–min) ≤0.4 °C (SD <0.1 °C)
Gradient function Yes (up to 20 °C span)
Optical system High-power broad-spectrum LED + CCD camera with fixed fiber optics (no scanning)
Channels 4-color multiplex (excitation/emission: 470/514, 533/572, 577/620, 645/697 nm)
Supported dyes SYBR Green I, FAM, VIC/HEX, Texas Red, Cy5, ResoLight, etc.
Dynamic range 10 orders of magnitude
Sensitivity Down to 1 copy
Runtime <40 min for 3-step 40-cycle PCR
Detection formats Intercalating dyes + Hydrolysis probes
Applications Absolute/relative quantification, melting curve analysis, endpoint genotyping, High-Resolution Melting (HRM)
Interface Integrated touchscreen (standalone) + optional PC connection
Dimensions 40 × 40 × 53 cm (W × D × H)
Weight Approx. 25–27 kg
Power 100–125 V AC, 50/60 Hz, ~600 W
Noise level ≤43 dB(A) during run

Additional information

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