Roche LightCycler 96 Real-Time PCR System
Used, tested₹250,000 + 18% GST
- Availability: arriving soon
- Condition: Used, tested
- Brand: Roche
- Model: LightCycler 96
Compact, mid-throughput real-time PCR system from Roche.
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Description
About this instrument
Compact, mid-throughput real-time PCR system from Roche. Features a high-precision 96-well Peltier block with gradient capability, 4-color optical detection via fixed fiber optics and CCD camera, and fast run times (<40 min for standard 40-cycle PCR). Ideal for gene expression, genotyping, HRM, and multiplex qPCR applications. Standalone touchscreen operation with intuitive software.
Specifications
| Brand | Roche |
|---|---|
| Model | LightCycler 96 |
| Condition | Used, tested |
| Format | 96-well block (supports 96-well plates and 8-tube strips) |
| Reaction volume | 10–50 µL |
| Thermal system | Peltier-based silver block |
| Max heating rate | 4.4 °C/s |
| Average cooling rate | 2.2 °C/s |
| Temperature range | 37–98 °C |
| Temperature accuracy | ±0.2 °C |
| Temperature uniformity | Range (max–min) ≤0.4 °C (SD <0.1 °C) |
| Gradient function | Yes (up to 20 °C span) |
| Optical system | High-power broad-spectrum LED + CCD camera with fixed fiber optics (no scanning) |
| Channels | 4-color multiplex (excitation/emission: 470/514, 533/572, 577/620, 645/697 nm) |
| Supported dyes | SYBR Green I, FAM, VIC/HEX, Texas Red, Cy5, ResoLight, etc. |
| Dynamic range | 10 orders of magnitude |
| Sensitivity | Down to 1 copy |
| Runtime | <40 min for 3-step 40-cycle PCR |
| Detection formats | Intercalating dyes + Hydrolysis probes |
| Applications | Absolute/relative quantification, melting curve analysis, endpoint genotyping, High-Resolution Melting (HRM) |
| Interface | Integrated touchscreen (standalone) + optional PC connection |
| Dimensions | 40 × 40 × 53 cm (W × D × H) |
| Weight | Approx. 25–27 kg |
| Power | 100–125 V AC, 50/60 Hz, ~600 W |
| Noise level | ≤43 dB(A) during run |
Additional information
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